Detection of human recombinant proBNP expressed mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells, 0.15 ug; Lane 1: pro-Brain Natriuretic Peptide Monoclonal Antibody (5B6) (Product # MA1-20622); Lane 2: pro-Brain Natriuretic Peptide Monoclonal Antibody (15F11) (Product # MA1-20626); Lane 3: proBNP Polyclonal Antibody [3G12] (Product # MA1-20886); Lane 4: pro-Brain Natriuretic Peptide Monoclonal Antibody (11D1) (Product # MA1-20584); Lane 5: pro-Brain Natriuretic Peptide Monoclonal Antibody (24E11) (Product # MA1-20631); Both pro-Brain Natriuretic Peptide Monoclonal Antibody (11D1) (Product # MA1-20584) and pro-Brain Natriuretic Peptide Monoclonal Antibody (24E11) (Product # MA1-20631)do not recognize glycosylated proBNP expressed in mammalian cells.